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Benchling Inc
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Benchling Inc
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Meso Scale Diagnostics LLC
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Benchling Inc
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Photonics Inc
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National Institute of Standards and Technology
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Photonics Inc
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Evergreen Labware
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Bio-Techne corporation
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Bio-Techne corporation
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Addgene inc
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Image Search Results
Journal: Frontiers in Plant Science
Article Title: Optimizing a tomato crocin biofactory by fine-tuning plant architecture
doi: 10.3389/fpls.2025.1730399
Figure Lengend Snippet: Targeted genes and constructs. (A) Representation of the exon regions of SP and SP5G genes, and the transcriptional unit of the hygromycin resistance gene ( HygR ). The positions of the designed guide RNAs (gRNAs) are indicated with arrows. (B) Schematic representation of the T-DNA of the constructs used to target SP , SP5G , and HygR with CRISPR/Cas9. nptII , kanamycin resistance gene; SP , self-pruning.
Article Snippet: The guide RNAs (gRNAs) targeting the tomato genes were designed for Streptococcus pyogenes Cas9 using
Techniques: Construct, CRISPR
Journal: Frontiers in Plant Science
Article Title: Optimizing a tomato crocin biofactory by fine-tuning plant architecture
doi: 10.3389/fpls.2025.1730399
Figure Lengend Snippet: CRISPR/Cas9 editing results. (A) Chromatograms of the selected sp # 4 and sp5g #6 edited lines. The gRNA sequences are underlined, and the PAM sequences are highlighted in red. The mismatches between the wild-type and mutated sequences are indicated in red in the mutated sequence. (B) Agarose gel of the PCR for HygR gene amplification. (C) Results of in vitro germination of hygr #6B seeds with and without hygromycin in the media. (D) Representation of the large deletion in hygr #6B line. TF, Tomaffron; SP , self-pruning.
Article Snippet: The guide RNAs (gRNAs) targeting the tomato genes were designed for Streptococcus pyogenes Cas9 using
Techniques: CRISPR, Sequencing, Agarose Gel Electrophoresis, Amplification, In Vitro